Journal: Biologics : Targets & Therapy

Article Title: MicroRNA-661 Suppresses Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells and Endothelial Cells by Targeting CCND2

doi: 10.2147/BTT.S592879

Figure Lengend Snippet: The negative correlation between miR-661 and CCND2 in pulmonary hypertension patients. ( A ) HPASMCs were overexpressed with miR-661, followed by RNA-seq analysis. The heatmap result showed the differentially expressed genes as indicated. ( B ) qRT-PCR was used to detect the expression level of miR-661 and CCND2 in serum of patients with pulmonary hypertension and healthy volunteers, then the correlation analysis were performed. ( C ) Statistics of KEGG pathway were performed after overexpressed with miR-661 in HPASMCs, the green highlighted text indicates that the cell cycle pathway was significantly inhibited.

Article Snippet: The human embryonic Kidney 293T cells (HEK293T), human pulmonary artery smooth muscle Cells (HPASMCs) and human pulmonary artery endothelial cells (HPAECs) were obtained from the American Type Culture Collection.

Techniques: RNA Sequencing, Quantitative RT-PCR, Expressing